Iterative Crystallography Service:Ubiquitin Carboxyl-terminal Hydrolase 7
http://www.creative-biostructure.com/Iterative-crystallography/Iterative-crystallography-CBCRY35.htm
蛋白水解酶测定
蛋白水解酶测定方法以荧光作为检测信号、以天然蛋白作为底物。该方法的关键之处是特异性地在蛋白N端、C端或其它位置定点标记上荧光染料,酶切这种天然折叠的蛋白底物会引起荧光信号的变化,从而测蛋白水解酶的活性
Iterative Crystallography Service:Pyrroline-5-carboxylate Reductase 1
http://www.creative-biostructure.com/Iterative-crystallography/Iterative-crystallography-CBCRY27.htm
Iterative Crystallography Service:Thioesterase superfamily member 2
http://www.creative-biostructure.com/Iterative-crystallography/Iterative-crystallography-CBCRY16.htm
Western Blot检测-蛋白水平技术服务
免疫印迹(Western戀氀漀琀)主要是利用电泳技术区分不同大小的蛋白,然后转移至固相支持物(PVDF膜,NC膜等)上,再根据抗原和抗体的特异性结合原理,通过化学发光或呈色等技术检测蛋白的表达。