NFIA抗体Applications
| In-house applications |
ELISA, WB, IHC, IP Application not tested? Please see our Guarantee. |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
|
Images
Prev1Next
 A431 cells were subjected to SDS PAGE followed by western blot with 11750-1-AP(NFIA antibody) at dilution of 1:1000 Immunohistochemical of paraffin-embedded human prostate cancer using 11750-1-AP(NFIA antibody) at dilution of 1:25 (under 10x lens) IP Result of anti-NFIA (IP:11750-1-AP, 4ug; Detection:11750-1-AP 1:500) with A431 cells lysate 2000ug.
|
| Positive WB detected in |
A431 cells, HeLa cells, Jurkat cells, L02 cells, mouse liver tissue |
| Positive IP detected in |
A431 cells |
| Positive IHC detected in |
Human prostate cancer tissue |
| Recommended dilution |
WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:10-1:100 |
NFIA抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information NFIA抗体
| Immunogen |
NFIA fusion protein ag2346 |
Full name |
Nuclear factor I/A |
Calculated molecular
weight |
498aa,55 kDa |
Observed molecular
weight |
60-70kd |
GenBank accession
number |
BC022264 |
Gene ID (NCBI) |
4774 |
| Gene symbol |
NFIA |
Synonyms |
CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein |
Background
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The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. Catalog# 11750-1-AP is a rabbit polyclonal antibody raised against N-terminal of human original NFIA. NFIA抗体
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